5-Methylcytosine (5mC) and 5-Hydroxymethylcytosine (5hmC) Enhance the DNA Binding of CREB1 to the C/EBP Half-Site Tetranucleotide GCAA.
Identifieur interne : 001421 ( Main/Exploration ); précédent : 001420; suivant : 0014225-Methylcytosine (5mC) and 5-Hydroxymethylcytosine (5hmC) Enhance the DNA Binding of CREB1 to the C/EBP Half-Site Tetranucleotide GCAA.
Auteurs : Khund Sayeed Syed [États-Unis] ; Ximiao He [États-Unis] ; Desiree Tillo [États-Unis] ; Jun Wang [États-Unis] ; Stewart R. Durell [États-Unis] ; Charles Vinson [États-Unis]Source :
- Biochemistry [ 1520-4995 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- analogues et dérivés : 5-Méthyl-cytosine.
- métabolisme : 5-Méthyl-cytosine, ADN, Protéine de liaison à l'élément de réponse à l'AMP cyclique, Protéines liant les séquences stimulatrices de type CCAAT.
- Animaux, Régions promotrices (génétique), Souris.
English descriptors
- KwdEn :
- MESH :
- chemical , analogs & derivatives : 5-Methylcytosine.
- chemical , metabolism : 5-Methylcytosine, CCAAT-Enhancer-Binding Proteins, Cyclic AMP Response Element-Binding Protein, DNA.
- Animals, Mice, Promoter Regions, Genetic.
Abstract
In human and mouse stem cells and brain, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) can occur outside of CG dinucleotides. Using protein binding microarrays (PBMs) containing 60-mer DNA probes, we evaluated the effect of 5mC and 5hmC on one DNA strand on the double-stranded DNA binding of the mouse B-ZIP transcription factors (TFs) CREB1, ATF1, and JUND. 5mC inhibited binding of CREB1 to the canonical CRE half-site |GTCA but enhanced binding to the C/EBP half-site |GCAA. 5hmC inhibited binding of CREB1 to all 8-mers except TGAT|GCAA, where binding is enhanced. We observed similar DNA binding patterns with ATF1, a closely related B-ZIP domain. In contrast, both 5mC and 5hmC inhibited binding of JUND. These results identify new DNA sequences that are well-bound by CREB1 and ATF1 only when they contain 5mC or 5hmC. Analysis of two X-ray structures examines the consequences of 5mC and 5hmC on DNA binding by CREB and FOS|JUN.
DOI: 10.1021/acs.biochem.6b00796
PubMed: 27951657
Affiliations:
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Durell</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Metabolism and ‡Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health , Room 3128, Building 37, Bethesda, Maryland 20892, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Metabolism and ‡Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health , Room 3128, Building 37, Bethesda, Maryland 20892</wicri:regionArea><wicri:noRegion>Maryland 20892</wicri:noRegion></affiliation></author><author><name sortKey="Vinson, Charles" sort="Vinson, Charles" uniqKey="Vinson C" first="Charles" last="Vinson">Charles Vinson</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Metabolism and ‡Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health , Room 3128, Building 37, Bethesda, Maryland 20892, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Metabolism and ‡Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health , Room 3128, Building 37, Bethesda, Maryland 20892</wicri:regionArea><wicri:noRegion>Maryland 20892</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biochemistry</title><idno type="eISSN">1520-4995</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>5-Methylcytosine (analogs & derivatives)</term><term>5-Methylcytosine (metabolism)</term><term>Animals</term><term>CCAAT-Enhancer-Binding Proteins (metabolism)</term><term>Cyclic AMP Response Element-Binding Protein (metabolism)</term><term>DNA (metabolism)</term><term>Mice</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>5-Méthyl-cytosine (analogues et dérivés)</term><term>5-Méthyl-cytosine (métabolisme)</term><term>ADN (métabolisme)</term><term>Animaux</term><term>Protéine de liaison à l'élément de réponse à l'AMP cyclique (métabolisme)</term><term>Protéines liant les séquences stimulatrices de type CCAAT (métabolisme)</term><term>Régions promotrices (génétique)</term><term>Souris</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>5-Methylcytosine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>5-Methylcytosine</term><term>CCAAT-Enhancer-Binding Proteins</term><term>Cyclic AMP Response Element-Binding Protein</term><term>DNA</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>5-Méthyl-cytosine</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>5-Méthyl-cytosine</term><term>ADN</term><term>Protéine de liaison à l'élément de réponse à l'AMP cyclique</term><term>Protéines liant les séquences stimulatrices de type CCAAT</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Mice</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Régions promotrices (génétique)</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In human and mouse stem cells and brain, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) can occur outside of CG dinucleotides. Using protein binding microarrays (PBMs) containing 60-mer DNA probes, we evaluated the effect of 5mC and 5hmC on one DNA strand on the double-stranded DNA binding of the mouse B-ZIP transcription factors (TFs) CREB1, ATF1, and JUND. 5mC inhibited binding of CREB1 to the canonical CRE half-site |GTCA but enhanced binding to the C/EBP half-site |GCAA. 5hmC inhibited binding of CREB1 to all 8-mers except TGAT|GCAA, where binding is enhanced. We observed similar DNA binding patterns with ATF1, a closely related B-ZIP domain. In contrast, both 5mC and 5hmC inhibited binding of JUND. These results identify new DNA sequences that are well-bound by CREB1 and ATF1 only when they contain 5mC or 5hmC. Analysis of two X-ray structures examines the consequences of 5mC and 5hmC on DNA binding by CREB and FOS|JUN.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Syed, Khund Sayeed" sort="Syed, Khund Sayeed" uniqKey="Syed K" first="Khund Sayeed" last="Syed">Khund Sayeed Syed</name></noRegion><name sortKey="Durell, Stewart R" sort="Durell, Stewart R" uniqKey="Durell S" first="Stewart R" last="Durell">Stewart R. Durell</name><name sortKey="He, Ximiao" sort="He, Ximiao" uniqKey="He X" first="Ximiao" last="He">Ximiao He</name><name sortKey="Tillo, Desiree" sort="Tillo, Desiree" uniqKey="Tillo D" first="Desiree" last="Tillo">Desiree Tillo</name><name sortKey="Vinson, Charles" sort="Vinson, Charles" uniqKey="Vinson C" first="Charles" last="Vinson">Charles Vinson</name><name sortKey="Wang, Jun" sort="Wang, Jun" uniqKey="Wang J" first="Jun" last="Wang">Jun Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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